Coding

Part:BBa_K5300011:Design

Designed by: Dini Piao   Group: iGEM24_CAU-China   (2024-09-21)


PPtase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 216
    Illegal NgoMIV site found at 759
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 430


Design Notes

Several base pairs were added after PPTase to reduce GC content and minimize non-specific primer binding


Source

Aetherobacter fasciculatus (SBSr002)

References